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Human Genome News Archive Edition

Human Genome News, March 1994; 5(6)

Resource: New Panels Available for Mapping Loci in the Mouse

The Jackson Laboratory is making available a new genetic-mapping resource of interspecific backcross DNA for mapping loci in the mouse. To facilitate the collective approach to generating a comprehensive map for the worldwide genome project, two DNA panels were established in microtiter format, one containing DNA from 94 N2 animals from the cross [C57BL/6J x M. spretus F1 females x C57BL/6J males (BSB Panel 1)] and another from 94 N2 animals from the reciprocal backcross [(C57BL/6J x SPRET/Ei) F1 females x SPRET/Ei males (BSS Panel 2)]. These DNA panels give wide access to mapping data and allow more research groups to participate simultaneously in mapping many types of loci. With a single microtiter plate for each panel set, complete data can be obtained readily for any polymorphism, and all new data result in map positions with highly significant linkage at a 1- to 5-cM resolution.

Large quantities (24 to 50 mg) of DNA were prepared from most tissues of each backcross animal. Initial characterization of the genetic maps of both panels has been completed. Massachusetts Institute of Technology single sequence length polymorphism (SSLP) markers [Dietrich et al., Genetics 131, 423-47 (1992)]; proviral loci [Frankel et al., J. Virol. 63, 1762-74 and 3810-21 (1989) and Genetics 124, 221-36 (1990)]; and several other sequence-defined genes [Takahashi and Ko, Genomics 16, 161-68 (1993)] were used to anchor these maps to other published maps. The BSB panel map (from the backcross to C57BL/6J) now contains 255 loci and is anchored by 50 SSLP and 32 gene sequence loci. The BSS panel map (from the backcross to SPRET/Ei) contains 666 loci and is anchored by 59 SSLP loci, 43 proviral loci, and 60 gene sequence loci. To obtain a high density of markers, motif-primed polymerase chain reaction (PCR) was used to "fingerprint" the panel DNAs [Birkenmeier et al., Mammalian Genome 3, 537-45 (1992)]. Since many loci are typed on only one of the panel sets, maps were constructed to represent each of the two panels. DNAs have been distributed to 38 laboratories around the world, and 23 investigators have contributed data to the maps. The Jackson Laboratory BC Panel Map Service is continuing to type additional anchor loci, and many other groups are adding new data to the map database. A complete preliminary report by Rowe et al. describing these results is in press at Mammalian Genome.

The backcross panel DNAs are available either as Southern blot filter sets (limited in 1994), aliquots of DNA for Southern blot analysis, or as aliquots of DNA to be diluted for use as PCR templates. Each backcross panel is in microtiter plate format with parental control DNAs in wells 1A and 1B and backcross N2 progeny DNAs in the remaining 94 wells. All data are catalogued by microtiter plate well position (e.g., 12H).

The Map Service is committed to providing technical support and genetic-mapping assistance as necessary to promote successful and efficient use of this DNA resource. A modest per-shipment charge is made for the use of the panels. [Contact: Lucy Rowe; The Jackson Laboratory; 600 Main Street; Bar Harbor, ME 04609 (207/288-3371, ext. 1687; Fax: -5079; Internet: lbr@aretha.jax.org). Return addresses should be included.]


[Lucy B. Rowe, Joseph H. Nadeau, Janan T. Eppig, and Edward H. Birkenmeier, The Jackson Laboratory]

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