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Human Genome News Archive Edition
Vol.9, No.3   July 1998

In this issue... 

Also available in pdf.

1997 Santa Fe Highlights

Human Genome Project Administration

In the News

Publications

Software and the Internet

Funding

Meeting Calendars & Acronyms

  • Genome and Biotechnology Meetings 
  • Training Courses and Workshops 
  • Acronyms

HGN archives and subscriptions   
HGP Information home 

JGI and "Bermuda-Quality" Sequence

The international sequencing community holds an annual meeting in Bermuda, sponsored by the Wellcome Trust, DOE, and NIH to set standards for DNA sequence with respect to cost, quality, timeliness of submission, and level of annotation. For details, see "Policies on Release of Human Genomic Sequence Data".

Standards for JGI meet or exceed those for "Bermuda-quality."

  • Sequencing targets: Megabase-sized (or larger) regions are the preferred targets to maximize biological impact.
  • Coverage: Goal is to complete sequence continuity across a target region, as feasible (no more than 1 gap in 200 kb on average).
  • Sequence accuracy: The acceptable error rate in finished sequence is 1 in 10,000 bases. JGI is using a rules-based approach for achieving this standard, which requires a minimum Phrap consensus value of 40 for each base and greater than 95% double-stranded coverage, with a minimum coverage of 2 high-quality reads with 1 read on the opposite strand.
  • Clone assembly verification: Two independent approaches will be used to verify accuracy of a clone's finished sequence.
  • Data submission and annotation: Minimum submission is the size of the starting clone, with 95% of sequence represented on both strands and all ambiguities resolved; sequences will be annotated to the extent feasible at the time of submission, largely automated; immediate release of finished annotated sequence.

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The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v9n3).

Human Genome Project 1990–2003

The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.

Human Genome News

Published from 1989 until 2002, this newsletter facilitated HGP communication, helped prevent duplication of research effort, and informed persons interested in genome research.