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Human Genome News Archive Edition
Vol.9, No.3   July 1998

In this issue... 

Also available in pdf.

1997 Santa Fe Highlights

Human Genome Project Administration

In the News


Software and the Internet


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JGI Clone Resource Task

Large-insert clones for sequencing are being mapped, selected, and validated at JGI member laboratories. Jan-Fang Cheng (LBNL) discussed JGI development of clone-based maps for production sequencing; other team leaders are Norman Doggett (LANL) and Anne Olsen (LLNL). Among the major goals are the complete closure and validation of existing maps of chromosomes 5, 16, and 19; selection of new mapping targets; and creation of pools and high-density filters of newly approved BAC libraries for STS screening.

Cheng observed that this year's challenge is to build templates for the FY 1999 ramp-up and beyond. Optimistic goals are to generate 70 Mb of contigs longer than 1 Mb, with associated restriction mapping data and over tenfold genome coverage. Summarizing the current map status of chromosomes 16, 19, and 5, Cheng reported that more than 70 Mb of contigs greater than 300 kb has been generated, with over 25 contigs (40 Mb) larger than 1 Mb.

Cheng pointed out that before JGI was established, the three laboratories used different clone types as well as different mapping approaches. Currently, the mapping plan calls for three autonomous map-production teams, although ideally one major production site will generate templates for PSF; a centralized clone repository will be set up with 1-Mb contigs prioritized for sequencing.

Breakdown for the clone resource task is resource production (15%), pilot R&D projects (15%), STS-content mapping (35%), and restriction mapping (35%). Cheng observed that, unlike sequencing strategies, mapping techniques evolve quickly and scientists want to retain flexibility.

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Human Genome Program, U.S. Department of Energy, Human Genome News (v9n3).

Human Genome Project 1990–2003

The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.

Human Genome News

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